TitleProteome-driven elucidation of adaptive responses to combined vitamin E and C deficiency in zebrafish.
Publication TypeJournal Article
Year of Publication2014
AuthorsMotorykin, I, Traber, MG, Tanguay, RL, Maier, CS
JournalJ Proteome Res
Date Published2014 Mar 07
KeywordsAdaptation, Physiological, Animals, Ascorbic Acid, Ascorbic Acid Deficiency, Chromatography, Liquid, Humans, Mass Spectrometry, Metabolic Networks and Pathways, Protein Interaction Mapping, Proteome, Tissue Extracts, Vitamin E, Vitamin E Deficiency, Zebrafish

The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism's response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.

Alternate JournalJ. Proteome Res.
PubMed ID24476500
PubMed Central IDPMC3993953
Grant ListS10 RR025628 / RR / NCRR NIH HHS / United States
P30ES000210 / ES / NIEHS NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
R01HD062109 / HD / NICHD NIH HHS / United States
S10RR025628 / RR / NCRR NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States