| Title | Interactions of Nitrosomonas europaea and Nitrobacter winogradskyi grown in co-culture. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Pérez, J, Buchanan, A, Mellbye, B, Ferrell, R, Chang, JH, Chaplen, F, Bottomley, PJ, Arp, DJ, Sayavedra-Soto, LA |
| Journal | Arch Microbiol |
| Volume | 197 |
| Issue | 1 |
| Pagination | 79-89 |
| Date Published | 2015 Jan |
| ISSN | 1432-072X |
| Keywords | Ammonia, Bacterial Load, Carbon Dioxide, Coculture Techniques, Culture Media, Energy Metabolism, Gene Expression, Genes, Bacterial, Microbial Interactions, Movement, Nitrites, Nitrobacter, Nitrosomonas europaea, Oxygen Consumption, Transcription, Genetic, Transcriptome |
| Abstract | Nitrosomonas europaea and Nitrobacter winogradskyi were grown singly and in co-culture in chemostats to probe for physiological differences between the two growth conditions. Co-culture growth medium containing 60 mM NH4 (+) resulted in a cell density (0.20-0.29 OD600) greater than the sum of the densities in single chemostat cultures, i.e., 0.09-0.14 OD600 for N. europaea with 60 mM NH4 (+)and 0.04-0.06 OD600 for N. winogradskyi with 60 mM NO2 (-). The NO2 (-)- and NH4 (+)-dependent O2 uptake rates, qRT-PCR, and microscopic observations indicated that in co-culture, N. europaea contributed ~0.20 OD600 (~80 %) and N. winogradskyi ~0.05 OD600 (~20 %). In co-culture, the transcriptomes showed that the mRNA levels of 773 genes in N. europaea (30.2 % of the genes) and of 372 genes in N. winogradskyi (11.8 % of the genes) changed significantly. Total cell growth and the analysis of the transcriptome revealed that in co-culture, N. europaea benefits more than N. winogradskyi. |
| DOI | 10.1007/s00203-014-1056-1 |
| Alternate Journal | Arch. Microbiol. |
| PubMed ID | 25362506 |
