|Title||The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Gütling, H, Bionaz, M, Sloboda, DM, Ehrlich, L, Braun, F, Gramzow, AK, Henrich, W, Plagemann, A, Braun, T|
|Date Published||2016 Aug|
RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene.